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Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) ELISA Kit

Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) ELISA Kit

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Specification

Product name Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA12 in samples. An antibody specific for SERPINA12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SERPINA12; OL-64; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 12; vaspin; visceral adipose-specific SERPIN;

Product Properties

Kit components • Rat Visceral adipose-specific serine protease inhibitor microplate
• Rat Visceral adipose-specific serine protease inhibitor standard
• Rat Visceral adipose-specific serine protease inhibitor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SERPINA12. No significant cross-reactivity or interference between Rat SERPINA12 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Visceral adipose tissue-derived serine protease inhibitor (vaspin) is an interesting novel adipocytokine with insulin-sensitizing effects. Some studies have suggested that vaspin could play an important role in the development of obesity and metabolic disorders. The induction of vaspin mRNA expression could represent a compensatory mechanism associated with obesity, severe insulin resistance and type 2 diabetes mellitus, however it is unclear whether a correlation exists between human vaspin serum levels and markers of insulin sensitivity and glucose or lipid metabolism. Vaspin serum concentrations have been shown to be lower in lean subjects and competitive sportsmen with long-term physical training, but they are increased with weight loss associated with a physical training programme.
Alternative SERPINA12; OL-64; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 12; vaspin; visceral adipose-specific SERPIN;
Accession Q8R4Z1

Image & description

Fig.1. Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) Standard Curve.

Fig.1. Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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