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Rat Tyrosine-protein kinase JAK3 (JAK3) ELISA Kit

Rat Tyrosine-protein kinase JAK3 (JAK3) ELISA Kit

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Specification

Product name Rat Tyrosine-protein kinase JAK3 (JAK3) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Tyrosine-protein kinase JAK3 (JAK3) ELISA Kit employs a two-site sandwich ELISA to quantitate Jak3 in samples. An antibody specific for Jak3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyJak3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Jak3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Jak3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative Jak3; JAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK; Janus kinase 3 (a protein tyrosine kinase; leukocyte); leukocyte Janus kinase; tyrosine-protein kinase JAK3

Product Properties

Kit components • Rat Tyrosine-protein kinase JAK3 microplate
• Rat Tyrosine-protein kinase JAK3 standard
• Rat Tyrosine-protein kinase JAK3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Tyrosine-protein kinase JAK3 (JAK3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat Jak3. No significant cross-reactivity or interference between Rat Jak3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background JAK3 encodes Janus kinase 3, a tyrosine kinase that belongs to the Janus family. JAK3 functions in signal transduction and interacts with members of the STAT (signal transduction and activators of transcription) family. JAK3 is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Since JAK3 expression is restricted mostly to hematopoietic cells, its role in cytokine signaling is thought to be more restricted than other JAKs. It is most commonly expressed in T cells and NK cells, but has been induced in other leukocytes, including monocytes. Jak3 is involved in signal transduction by receptors that employ the common gamma chain (γC) of the type I cytokine receptor family .
Alternative Jak3; JAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK; Janus kinase 3 (a protein tyrosine kinase; leukocyte); leukocyte Janus kinase; tyrosine-protein kinase JAK3
Accession Q63272

Image & description

Fig.1. Rat Tyrosine-protein kinase JAK3 (JAK3) Standard Curve.

Fig.1. Rat Tyrosine-protein kinase JAK3 (JAK3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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