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Rat Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) ELISA Kit

Rat Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) ELISA Kit

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Specification

Product name Rat Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF1B in samples. An antibody specific for TNFRSF1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNFRSF1B; CD120b; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; p75; p75TNFR; p75 TNF receptor; soluble TNFR1B variant 1; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; tu

Product Properties

Kit components • Rat Tumor necrosis factor receptor superfamily member 1B microplate
• Rat Tumor necrosis factor receptor superfamily member 1B standard
• Rat Tumor necrosis factor receptor superfamily member 1B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNFRSF1B. No significant cross-reactivity or interference between Rat TNFRSF1B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TNFRSF1B is a human gene. The protein encoded by this gene is a member of the Tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways.
Gene ID 156767
Alternative TNFRSF1B; CD120b; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; p75; p75TNFR; p75 TNF receptor; soluble TNFR1B variant 1; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; tu
Accession Q80WY6

Image & description

Fig.1. Rat Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) Standard Curve.

Fig.1. Rat Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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