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Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) ELISA Kit

Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) ELISA Kit

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Specification

Product name Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) ELISA Kit employs a two-site sandwich ELISA to quantitate PUS1 in samples. An antibody specific for PUS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPUS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PUS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PUS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PUS1; MGC11268; pseudouridine synthase 1; tRNA uridine isomerase I

Product Properties

Kit components • Rat TRNA pseudouridine synthase A, mitochondrial microplate
• Rat TRNA pseudouridine synthase A, mitochondrial standard
• Rat TRNA pseudouridine synthase A, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PUS1. No significant cross-reactivity or interference between Rat PUS1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PUS1 converts uridine into pseudouridine after the nucleotide has been incorporated into RNA. Pseudouridine may have a functional role in tRNAs and may assist in the peptidyl transfer reaction of rRNAs PUS1-1 encodes a protein with an N-terminal mitochondrial leader peptide. Western blot analysis detected full-length PUS1-1 at an apparent molecular mass of 47 kD, and the mature protein, lacking the mitochondrial localization signal, was about 37 kD. The PUS1-2 and PUS1-3 transcripts differ in the 5-prime untranslated region but encode the same protein that begins with an internal methionine (met29) relative to PUS1-1. Immunofluorescence localized PUS1-1 to mitochondria and PUS1-2 and PUS1-3 to nuclei.
Gene ID 304567
Alternative PUS1; MGC11268; pseudouridine synthase 1; tRNA uridine isomerase I
Accession Q4KM92

Image & description

Fig.1. Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) Standard Curve.

Fig.1. Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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