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Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) ELISA Kit

Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) ELISA Kit

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Specification

Product name Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAP2 in samples. An antibody specific for TAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TAP2; DAAP-57C1.2; ABC18; ABCB3; APT2; D6S217E; PSF2; RING11; ABC transporter; MHC 2; ATP-binding cassette; sub-family B (MDR/TAP); member 3; antigen peptide transporter 2; peptide supply factor 2; peptide tr

Product Properties

Kit components • Rat Transporter 2, ATP-binding cassette, sub-family B microplate
• Rat Transporter 2, ATP-binding cassette, sub-family B standard
• Rat Transporter 2, ATP-binding cassette, sub-family B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TAP2. No significant cross-reactivity or interference between Rat TAP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The membrane-associated protein encoded by TAP2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. [provided by RefSeq, Feb 2014]
Gene ID 24812
Alternative TAP2; DAAP-57C1.2; ABC18; ABCB3; APT2; D6S217E; PSF2; RING11; ABC transporter; MHC 2; ATP-binding cassette; sub-family B (MDR/TAP); member 3; antigen peptide transporter 2; peptide supply factor 2; peptide tr
Accession P36372

Image & description

Fig.1. Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) Standard Curve.

Fig.1. Rat Transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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