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Rat Transmembrane protein 176A (TMEM176A) ELISA Kit

Rat Transmembrane protein 176A (TMEM176A) ELISA Kit

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Specification

Product name Rat Transmembrane protein 176A (TMEM176A) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Transmembrane protein 176A (TMEM176A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM176A in samples. An antibody specific for TMEM176A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM176A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM176A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM176A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMEM176A; GS188; HCA112; hepatocellular carcinoma-associated antigen 112; likley ortholog of mouse GS188

Product Properties

Kit components • Rat Transmembrane protein 176A microplate
• Rat Transmembrane protein 176A standard
• Rat Transmembrane protein 176A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Transmembrane protein 176A (TMEM176A) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TMEM176A. No significant cross-reactivity or interference between Rat TMEM176A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background By serologic analysis of recombinant cDNA expression libraries (SEREX) from 4 hepatocellular carcinoma patients, Wang et al. (2002) cloned HCA112. The deduced protein contains 235 amino acids. RT-PCR detected HCA112 expression in human kidney, lung, and pancreas, but not in any other tissue examined. Nakajima et al. (2002) cloned the mouse homolog of HCA112, Gs188. The deduced 244-amino acid protein has a calculated molecular mass of 26.6 kD and contains 4 putative hydrophobic domains. Northern blot analysis detected Gs188 expression in mouse kidney, lung, and spleen; little to no expression was detected in other tissues examined.
Gene ID 297077
Alternative TMEM176A; GS188; HCA112; hepatocellular carcinoma-associated antigen 112; likley ortholog of mouse GS188
Accession Q4G068

Image & description

Fig.1. Rat Transmembrane protein 176A (TMEM176A) Standard Curve.

Fig.1. Rat Transmembrane protein 176A (TMEM176A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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