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Rat Transmembrane protein 165 (TMEM165) ELISA Kit

Rat Transmembrane protein 165 (TMEM165) ELISA Kit

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Specification

Product name Rat Transmembrane protein 165 (TMEM165) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Transmembrane protein 165 (TMEM165) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM165 in samples. An antibody specific for TMEM165 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM165 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM165 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM165 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMEM165; TMPT27; TPARL; TPA regulated locus

Product Properties

Kit components • Rat Transmembrane protein 165 microplate
• Rat Transmembrane protein 165 standard
• Rat Transmembrane protein 165 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Transmembrane protein 165 (TMEM165) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TMEM165. No significant cross-reactivity or interference between Rat TMEM165 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TMEM165, Belongs to the UPF0016 (TC 9.B.26) family.The Clock gene encodes a basic helix-loop-helix (bHLH)-PAS transcription factor that regulates circadian rhythms in mice. This region contains the complete loci for the Clock and Tpardl (pFT27) genes, as well as the 3' partial locus of the Neuromedin U gene; sequence analysis also suggests the presence of two previously unidentified genes. Finally, a new BAC transgenic line indicates that the genomic region that is sufficient for rescue of the Clock mutant phenotype is no greater than 120 kb and tightly flanks the 3' end of the Clock gene.
Gene ID 364137
Alternative TMEM165; TMPT27; TPARL; TPA regulated locus
Accession Q4V899

Image & description

Fig.1. Rat Transmembrane protein 165 (TMEM165) Standard Curve.

Fig.1. Rat Transmembrane protein 165 (TMEM165) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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