|Product name||Rat Transmembrane 6 superfamily member 2 (TM6SF2) ELISA Kit|
|Applications notes||This Rat Transmembrane 6 superfamily member 2 (TM6SF2) ELISA Kit employs a two-site sandwich ELISA to quantitate TM6SF2 in samples. An antibody specific for TM6SF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM6SF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM6SF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM6SF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Transmembrane 6 superfamily member 2 microplate
• Rat Transmembrane 6 superfamily member 2 standard
• Rat Transmembrane 6 superfamily member 2 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Transmembrane 6 superfamily member 2 (TM6SF2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TM6SF2. No significant cross-reactivity or interference between Rat TM6SF2 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||TM9SF2 contains a putative signal sequence, a hydrophilic N-terminal region, and 9 predicted C-terminal transmembrane domains. TM9SF2 shares 35% amino acid sequence identity with the S. cerevisiae Emp70 protein. By indirect immunofluorescence microscopy, recombinant TM9SF2 appeared to be localized to endosomes by virtue of its apparent colocalization with transferrin receptors and some mannose 6-phosphate receptors; TM9SF2 was not detected in the plasma membrane or in the Golgi apparatus. Northern blot analysis detected an approximately 3.2-kb TM9SF2 transcript in all human tissues tested, with the highest expression in pancreas, high expression in kidney, lower expression in heart, brain, skeletal muscle, and placenta, and the lowest expression in lung and liver.|
Fig.1. Rat Transmembrane 6 superfamily member 2 (TM6SF2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.