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Rat Transmembrane 4 L6 family member 4 (TM4SF4) ELISA Kit

Rat Transmembrane 4 L6 family member 4 (TM4SF4) ELISA Kit

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Specification

Product name Rat Transmembrane 4 L6 family member 4 (TM4SF4) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Transmembrane 4 L6 family member 4 (TM4SF4) ELISA Kit employs a two-site sandwich ELISA to quantitate TM4SF4 in samples. An antibody specific for TM4SF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM4SF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM4SF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM4SF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TM4SF4; FLJ31015; ILTMP; il-TMP; intestinal and liver (il) tetraspan membrane protein; transmembrane 4 superfamily member 4

Product Properties

Kit components • Rat Transmembrane 4 L6 family member 4 microplate
• Rat Transmembrane 4 L6 family member 4 standard
• Rat Transmembrane 4 L6 family member 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Transmembrane 4 L6 family member 4 (TM4SF4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TM4SF4. No significant cross-reactivity or interference between Rat TM4SF4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TM4SF4 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that can regulate cell proliferation. The use of alternate polyadenylation sites has been found for this gene. Sequence analysis predicted that the 202-amino acid tetraspan membrane protein, which is 50% identical to TM4SF1, contains 4 hydrophobic transmembrane domains and a hydrophilic region between domains 3 and 4 that contains 2 potential N-linked glycosylation sites.
Gene ID 116467
Alternative TM4SF4; FLJ31015; ILTMP; il-TMP; intestinal and liver (il) tetraspan membrane protein; transmembrane 4 superfamily member 4
Accession Q9EQL5

Image & description

Fig.1. Rat Transmembrane 4 L6 family member 4 (TM4SF4) Standard Curve.

Fig.1. Rat Transmembrane 4 L6 family member 4 (TM4SF4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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