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Rat Transforming growth factor β3 (TGF-β3) ELISA Kit

Rat Transforming growth factor β3 (TGF-β3) ELISA Kit

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Specification

Product name Rat Transforming growth factor β3 (TGF-β3) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Transforming growth factor β3 (TGF-β3) ELISA Kit employs a two-site sandwich ELISA to quantitate TGF-β3 in samples. An antibody specific for TGF-β3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGF-β3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-β3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-β3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TGF-β3; ARVD; FLJ16571; TGF-beta3;

Product Properties

Kit components • Rat Transforming growth factor β3 microplate
• Rat Transforming growth factor β3 standard
• Rat Transforming growth factor β3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Transforming growth factor β3 (TGF-β3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TGF-β3. No significant cross-reactivity or interference between Rat TGF-β3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TGF-β3 gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This protein is involved in embryogenesis and cell differentiation, and may play a role in wound healing.
Alternative TGF-β3; ARVD; FLJ16571; TGF-beta3;

Image & description

Fig.1. Rat Transforming growth factor β3 (TGF-β3) Standard Curve.

Fig.1. Rat Transforming growth factor β3 (TGF-β3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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