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Rat Trace amine-associated receptor 2 (TAAR2) ELISA Kit

Rat Trace amine-associated receptor 2 (TAAR2) ELISA Kit

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Specification

Product name Rat Trace amine-associated receptor 2 (TAAR2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Trace amine-associated receptor 2 (TAAR2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAAR2 in samples. An antibody specific for TAAR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAAR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAAR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAAR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TAAR2; GPR58; G protein-coupled receptor 58; OTTHUMP00000017225

Product Properties

Kit components • Rat Trace amine-associated receptor 2 microplate
• Rat Trace amine-associated receptor 2 standard
• Rat Trace amine-associated receptor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Trace amine-associated receptor 2 (TAAR2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TAAR2. No significant cross-reactivity or interference between Rat TAAR2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Trace amine-associated receptor 2 (TAAR2), formerly known as G protein-coupled receptor 58 (GPR58), is a protein that in humans is encoded by the TAAR2 gene.TAAR2 is coexpressed with Gα proteins; however, as of February 2017, its signal transduction mechanisms have not been determined. Human TAAR2 (hTAAR2) is expressed in the cerebellum, olfactory sensory neurons in the olfactory epithelium, and leukocytes (i.e., white blood cells), among other tissues. hTAAR1 and hTAAR2 are both required for white blood cell activation by trace amines in granulocytes.Phenethylamine, para-tyramine, and 3-iodothyronamine are full agonists of hTAAR2 with EC50 values of 0.43 ± 0.05 nM, 0.52 ± 0.05 nM, and 0.25 ± 0.04 nM respectively. A single nucleotide polymorphism nonsense mutation of the TAAR2 gene is associated with schizophrenia. TAAR2 is a probable pseudogene in 10–15% of Asians as a result of a polymorphism that produces a premature stop codon at amino acid 168.
Gene ID 294121
Alternative TAAR2; GPR58; G protein-coupled receptor 58; OTTHUMP00000017225
Accession Q5QD25

Image & description

Fig.1. Rat Trace amine-associated receptor 2 (TAAR2) Standard Curve.

Fig.1. Rat Trace amine-associated receptor 2 (TAAR2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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