|Product name||Rat Thyroglobulin IgG antibody (TAb-IgG) ELISA Kit|
|Applications notes||This Rat Thyroglobulin IgG antibody (TAb-IgG) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TAb-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated TAb-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled TAb-IgG and unlabeled TAb-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TAb-IgG in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Thyroglobulin IgG antibody microplate
• Rat Thyroglobulin IgG antibody standard
• Rat Thyroglobulin IgG antibody detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Thyroglobulin IgG antibody (TAb-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TAb-IgG. No significant cross-reactivity or interference between Rat TAb-IgG and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Thyroglobulin (Tg) is a 660 kDa, dimeric protein produced by and used entirely within the thyroid gland. In earlier literature, Tg was referred to as "colloid".Thyroglobulin should not be confused with Thyroxine-binding globulin, a carrier protein responsible for carrying the thyroid hormones in the blood.Tg is used by the thyroid gland to produce the thyroid hormones thyroxine (T4) and triiodothyronine (T3). The active form of triiodothyronine, 3, 5, 3' triiodothyronine, is produced both within the thyroid gland and periphery by 5'-deiodinase (which has been referred to as tetraiodothyronine 5' deiodinase.)Thyroglobulin levels in the blood can be used as a tumor marker for certain kinds of thyroid cancer. Thyroglobulin levels in the blood can also be elevated in cases of Graves' disease.|
Fig.1. Rat Thyroglobulin IgG antibody (TAb-IgG) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.