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Rat Thy-1 membrane glycoprotein (THY1) ELISA Kit

Rat Thy-1 membrane glycoprotein (THY1) ELISA Kit

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Specification

Product name Rat Thy-1 membrane glycoprotein (THY1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Thy-1 membrane glycoprotein (THY1) ELISA Kit employs a two-site sandwich ELISA to quantitate THY1 in samples. An antibody specific for THY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTHY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for THY1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of THY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative THY1; CD90; FLJ33325; Thy-1 T-cell antigen; Thy-1 membrane glycoprotein

Product Properties

Kit components • Rat Thy-1 membrane glycoprotein microplate
• Rat Thy-1 membrane glycoprotein standard
• Rat Thy-1 membrane glycoprotein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Thy-1 membrane glycoprotein (THY1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat THY1. No significant cross-reactivity or interference between Rat THY1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Thy-1 or CD90 (Cluster of Differentiation 90) is a 25 ndash 37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.Thy-1 was discovered in early 1960s during the search for heterologous antisera against mouse leukemia cells. It was originally named theta, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in thymocytes.
Gene ID 24832
Alternative THY1; CD90; FLJ33325; Thy-1 T-cell antigen; Thy-1 membrane glycoprotein
Accession P01830

Image & description

Fig.1. Rat Thy-1 membrane glycoprotein (THY1) Standard Curve.

Fig.1. Rat Thy-1 membrane glycoprotein (THY1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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