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Rat Tartrate-resistant acid phosphatase type 5 (ACP5) ELISA Kit

Rat Tartrate-resistant acid phosphatase type 5 (ACP5) ELISA Kit

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Specification

Product name Rat Tartrate-resistant acid phosphatase type 5 (ACP5) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Tartrate-resistant acid phosphatase type 5 (ACP5) ELISA Kit employs a two-site sandwich ELISA to quantitate ACP5 in samples. An antibody specific for ACP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACP5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ACP5; MGC117378; TRAP; TrATPase; tartrate resistant acid phosphatase 5; tartrate-resistant acid ATPase

Product Properties

Kit components • Rat Tartrate-resistant acid phosphatase type 5 microplate
• Rat Tartrate-resistant acid phosphatase type 5 standard
• Rat Tartrate-resistant acid phosphatase type 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Tartrate-resistant acid phosphatase type 5 (ACP5) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ACP5. No significant cross-reactivity or interference between Rat ACP5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Type 5 acid phosphatase is a glycosylated monomeric metalloenzyme expressed in mammals. It has a molecular weight of approximately 35kDa, a basic isoelectric point (7.6-9.5) , and optimal activity in acidic conditions. TRAP is synthesized as latent proenzyme and activated by proteolytic cleavage and reduction. It is differentiated by other mammalian acid phosphatases by its resistance to inhibition by tartrate, molecular weights and characteristic purple colour. The mechanism of phosphate ester hydrolysis by TRAP is through a nucleophilic attack mechanism, whereby, catalysis occurs with the binding of a phosphate-substrate to the Fe2+ in the active site of TRAP. This is then followed by a nucleophilic attack by a hydroxide ligand on the bound phosphorus atom, resulting in cleavage of the phosphate ester bond and production of an alcohol.
Gene ID 100008599
Alternative ACP5; MGC117378; TRAP; TrATPase; tartrate resistant acid phosphatase 5; tartrate-resistant acid ATPase
Accession O97860

Image & description

Fig.1. Rat Tartrate-resistant acid phosphatase type 5 (ACP5) Standard Curve.

Fig.1. Rat Tartrate-resistant acid phosphatase type 5 (ACP5) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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