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Rat Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit

Rat Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit

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Specification

Product name Rat Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKAP1 in samples. An antibody specific for MAPKAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPKAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAPKAP1; RP11-269P11.1; JC310; MGC2745; MIP1; SIN1; SIN1b; SIN1g; MEKK2-interacting protein 1; OTTHUMP00000022141; SAPK-interacting protein 1; ras inhibitor MGC2745; stress-activated map kinase interacting prote

Product Properties

Kit components • Rat Target of rapamycin complex 2 subunit MAPKAP1 microplate
• Rat Target of rapamycin complex 2 subunit MAPKAP1 standard
• Rat Target of rapamycin complex 2 subunit MAPKAP1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAPKAP1. No significant cross-reactivity or interference between Rat MAPKAP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Target of rapamycin complex 2 subunit MAPKAP1 is a protein similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. The deduced 522-amino acid protein has a nuclear localization signal, 2 bipartite nuclear localization signals, a peroxisomal targeting signal, and a PEST motif for rapid protein degradation. Comparison of SIN1 with homologs from various species revealed a short, highly conserved region, designated Box1, located within a larger conserved domain, designated CRIM (conserved region in middle).
Gene ID 296648
Alternative MAPKAP1; RP11-269P11.1; JC310; MGC2745; MIP1; SIN1; SIN1b; SIN1g; MEKK2-interacting protein 1; OTTHUMP00000022141; SAPK-interacting protein 1; ras inhibitor MGC2745; stress-activated map kinase interacting prote
Accession Q6AYF1

Image & description

Fig.1. Rat Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) Standard Curve.

Fig.1. Rat Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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