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Rat Syndecan-2 (SDC2) ELISA Kit

Rat Syndecan-2 (SDC2) ELISA Kit

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Specification

Product name Rat Syndecan-2 (SDC2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Syndecan-2 (SDC2) ELISA Kit employs a two-site sandwich ELISA to quantitate SDC2 in samples. An antibody specific for SDC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SDC2; HSPG; HSPG1; SYND2; cell surface-associated heparan sulfate proteoglycan 1; fibroglycan; heparan sulfate proteoglycan 1; cell surface-associated; heparan sulfate proteoglycan core protein; syndecan prot

Product Properties

Kit components • Rat Syndecan-2 microplate
• Rat Syndecan-2 standard
• Rat Syndecan-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Syndecan-2 (SDC2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SDC2. No significant cross-reactivity or interference between Rat SDC2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Syndecan-2 is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-2 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-2 expression has been detected in several different tumor types.By Southern hybridization to a panel of human-mouse somatic cell hybrid DNA and by in situ hybridization, Marynen et al. (1989) showed that the heparan sulfate proteoglycan core protein maps to 8q22-q24.
Gene ID 25615
Alternative SDC2; HSPG; HSPG1; SYND2; cell surface-associated heparan sulfate proteoglycan 1; fibroglycan; heparan sulfate proteoglycan 1; cell surface-associated; heparan sulfate proteoglycan core protein; syndecan prot
Accession P34900

Image & description

Fig.1. Rat Syndecan-2 (SDC2) Standard Curve.

Fig.1. Rat Syndecan-2 (SDC2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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