Login Register


$ 0

Rat Synaptojanin-2 (SYNJ2) ELISA Kit

Rat Synaptojanin-2 (SYNJ2) ELISA Kit

Views(259) Publications(0) Catalog no(KTE100151)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)


Product name Rat Synaptojanin-2 (SYNJ2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Synaptojanin-2 (SYNJ2) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNJ2 in samples. An antibody specific for SYNJ2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNJ2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNJ2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNJ2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SYNJ2; RP11-266C7.2; INPP5H; KIAA0348; MGC44422; inositol phosphate 5 -phosphatase 2 (synaptojanin 2)

Product Properties

Kit components • Rat Synaptojanin-2 microplate
• Rat Synaptojanin-2 standard
• Rat Synaptojanin-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Synaptojanin-2 (SYNJ2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SYNJ2. No significant cross-reactivity or interference between Rat SYNJ2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Synaptojanin-2 is a ubiquitously expressed inositol polyphosphate 5-phosphatase. The longest deduced protein, designated Synj2-alpha, contains 1,216 amino acids and has a calculated molecular mass of 140 kD. Synj2 contains an N-terminal Sac1 (SACM1L) homology domain, a central inositol 5-prime-phosphatase domain, and a C-terminal proline-rich region. Synj2 variants arise from the use of 2 possible initiation sites and at least 6 different exons encoding C-terminal domains. Initiation from the alternative start sites results in isoforms that vary in the presence of 84 amino acids in the N-terminal part of the Sac1 homology domain. Database analysis indicated the presence of a third putative initiation site in human SYNJ2, resulting in a protein that lacks most of the Sac1 homology domain.
Gene ID 84018
Alternative SYNJ2; RP11-266C7.2; INPP5H; KIAA0348; MGC44422; inositol phosphate 5 -phosphatase 2 (synaptojanin 2)
Accession O55207

Image & description

Fig.1. Rat Synaptojanin-2 (SYNJ2) Standard Curve.

Fig.1. Rat Synaptojanin-2 (SYNJ2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications


There are no reviews yet.

Be the first to review “Rat Synaptojanin-2 (SYNJ2) ELISA Kit”