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Rat Stromal cell-derived factor 1 alpha (SDF1a) ELISA Kit

Rat Stromal cell-derived factor 1 alpha (SDF1a) ELISA Kit

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Specification

Product name Rat Stromal cell-derived factor 1 alpha (SDF1a) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Stromal cell-derived factor 1 alpha (SDF1a) ELISA Kit employs a two-site sandwich ELISA to quantitate SDF1a in samples. An antibody specific for SDF1a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDF1a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDF1a is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDF1a bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SDF1a; CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12

Product Properties

Kit components • Rat Stromal cell-derived factor 1 alpha microplate
• Rat Stromal cell-derived factor 1 alpha standard
• Rat Stromal cell-derived factor 1 alpha detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Stromal cell-derived factor 1 alpha (SDF1a) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SDF1a. No significant cross-reactivity or interference between Rat SDF1a and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12).SDF-1 is produced in two forms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, by alternate splicing of the same gene. SDF-1β is synthesized as a 93 amino acid (aa) precursor that contains a 21 aa signal sequence and a 72 aa mature region. The mature molecule exhibits a typical three antiparallel β-strand chemokine-like fold. There are no potential N-linked glycosylation sites. N-terminal aa ’s 1-8 form a receptor binding site, while aa’s 1 and 2 (Lys-Pro) are involved in receptor activation. The C-terminus is likely associated with heparin binding. SDF-1β circulates and undergoes proteolytic processing. SDF-1β are reported to be a monomers at neutral pH and physiologic ionic strength.
Alternative SDF1a; CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12

Image & description

Fig.1. Rat Stromal cell-derived factor 1 alpha (SDF1a) Standard Curve.

Fig.1. Rat Stromal cell-derived factor 1 alpha (SDF1a) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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