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Rat Stem cell factor/mast cell growth factor (SCF/MGF) ELISA Kit

Rat Stem cell factor/mast cell growth factor (SCF/MGF) ELISA Kit

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Specification

Product name Rat Stem cell factor/mast cell growth factor (SCF/MGF) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Stem cell factor/mast cell growth factor (SCF/MGF) ELISA Kit employs a two-site sandwich ELISA to quantitate KITLG in samples. An antibody specific for KITLG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKITLG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KITLG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KITLG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative KITLG; DKFZp686F2250; FPH2; KL-1; Kitl; MGF; SCF; SF; SHEP7; familial progressive hyperpigmentation 2; mast cell growth factor; steel factor; stem cell factor

Product Properties

Kit components • Rat Stem cell factor/mast cell growth factor microplate
• Rat Stem cell factor/mast cell growth factor standard
• Rat Stem cell factor/mast cell growth factor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Stem cell factor/mast cell growth factor (SCF/MGF) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KITLG. No significant cross-reactivity or interference between Rat KITLG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Stem cell factor (SCF;MGF) is a cytokine which binds CD117 (c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E(burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid). SCF, along with bFGF (basic fibroblast growth factor) and LIF (leukemia inhibitory factor), prevents spontaneous differentiation of primitive embryonic stem cells in cell culture.
Gene ID 60427
Alternative KITLG; DKFZp686F2250; FPH2; KL-1; Kitl; MGF; SCF; SF; SHEP7; familial progressive hyperpigmentation 2; mast cell growth factor; steel factor; stem cell factor
Accession P21581

Image & description

Fig.1. Rat Stem cell factor/mast cell growth factor (SCF/MGF) Standard Curve.

Fig.1. Rat Stem cell factor/mast cell growth factor (SCF/MGF) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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