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Rat Sphingosine kinase 1 (SPHK1) ELISA Kit

Rat Sphingosine kinase 1 (SPHK1) ELISA Kit

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Specification

Product name Rat Sphingosine kinase 1 (SPHK1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Sphingosine kinase 1 (SPHK1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPHK1 in samples. An antibody specific for SPHK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPHK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPHK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPHK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPHK1; SPHK;

Product Properties

Kit components • Rat Sphingosine kinase 1 microplate
• Rat Sphingosine kinase 1 standard
• Rat Sphingosine kinase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Sphingosine kinase 1 (SPHK1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SPHK1. No significant cross-reactivity or interference between Rat SPHK1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by SPHK1 gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.
Gene ID 170897
Alternative SPHK1; SPHK;
Accession Q91V26

Image & description

Fig.1. Rat Sphingosine kinase 1 (SPHK1) Standard Curve.

Fig.1. Rat Sphingosine kinase 1 (SPHK1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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