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Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) ELISA Kit

Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) ELISA Kit

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Specification

Product name Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SGPP1 in samples. An antibody specific for SGPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySGPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SGPP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SGPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SGPP1; SPPase1; sphingosine-1-phosphatase

Product Properties

Kit components • Rat Sphingosine-1-phosphate phosphatase 1 microplate
• Rat Sphingosine-1-phosphate phosphatase 1 standard
• Rat Sphingosine-1-phosphate phosphatase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SGPP1. No significant cross-reactivity or interference between Rat SGPP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides . The deduced 430-amino acid mouse protein shares 17% amino acid identity with yeast Lbp1. The deduced 441-amino acid protein shares 76% amino acid identity with its mouse homolog. Transfection of HEK293 or Chinese hamster ovary (CHO) cells with SGPP1 resulted in marked increase in SGPP1 activity in membrane fractions. SGPP1 was highly specific toward long-chain sphingoid base phosphates and degraded S1P, dihydro-S1P, and phyto-S1P. SGPP1 activity was independent of any cation requirements, including Mg(2+), and was not inhibited by EDTA but was inhibited by Zn(2+).
Alternative SGPP1; SPPase1; sphingosine-1-phosphatase
Accession Q99P55

Image & description

Fig.1. Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) Standard Curve.

Fig.1. Rat Sphingosine-1-phosphate phosphatase 1 (SGPP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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