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Rat Sphingolipid delta (DEGS2) ELISA Kit

Rat Sphingolipid delta (DEGS2) ELISA Kit

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Specification

Product name Rat Sphingolipid delta (DEGS2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Sphingolipid delta (DEGS2) ELISA Kit employs a two-site sandwich ELISA to quantitate DEGS2 in samples. An antibody specific for DEGS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDEGS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEGS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEGS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DEGS2; C14orf66; DES2; FADS8; degenerative spermatocyte homolog 2; lipid desaturase; sphingolipid C4-hydroxylase/delta 4-desaturase; sphingolipid delta 4 desaturase/C-4 hydroxylase; sphingolipid delta(4)-desa

Product Properties

Kit components • Rat Sphingolipid delta microplate
• Rat Sphingolipid delta standard
• Rat Sphingolipid delta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Sphingolipid delta (DEGS2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DEGS2. No significant cross-reactivity or interference between Rat DEGS2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DEGS2 encodes a bifunctional enzyme that is involved in the biosynthesis of phytosphingolipids in human skin and in other phytosphingolipid-containing tissues. This enzyme can act as a sphingolipid delta(4)-desaturase, and also as a sphingolipid C4-hydroxylase.
Gene ID 314438
Alternative DEGS2; C14orf66; DES2; FADS8; degenerative spermatocyte homolog 2; lipid desaturase; sphingolipid C4-hydroxylase/delta 4-desaturase; sphingolipid delta 4 desaturase/C-4 hydroxylase; sphingolipid delta(4)-desa
Accession Q564G3

Image & description

Fig.1. Rat Sphingolipid delta (DEGS2) Standard Curve.

Fig.1. Rat Sphingolipid delta (DEGS2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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