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Rat Sorbin and SH3 domain-containing protein 2 (SORBS2) ELISA Kit

Rat Sorbin and SH3 domain-containing protein 2 (SORBS2) ELISA Kit

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Specification

Product name Rat Sorbin and SH3 domain-containing protein 2 (SORBS2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Sorbin and SH3 domain-containing protein 2 (SORBS2) ELISA Kit employs a two-site sandwich ELISA to quantitate SORBS2 in samples. An antibody specific for SORBS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySORBS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORBS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORBS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SORBS2; ARGBP2; FLJ93447; KIAA0777; PRO0618; Arg binding protein 2; Arg/Abl interacting protein; Arg/Abl-interacting protein 2; OTTHUMP00000211726

Product Properties

Kit components • Rat Sorbin and SH3 domain-containing protein 2 microplate
• Rat Sorbin and SH3 domain-containing protein 2 standard
• Rat Sorbin and SH3 domain-containing protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Sorbin and SH3 domain-containing protein 2 (SORBS2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SORBS2. No significant cross-reactivity or interference between Rat SORBS2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. They interact with the Arg/Abl binding proteins via the SH3 domains present in the carboxy end of the latter group of proteins. This gene encodes the sorbin and SH3 domain containing 2 protein. It has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that it functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Gene ID 114901
Alternative SORBS2; ARGBP2; FLJ93447; KIAA0777; PRO0618; Arg binding protein 2; Arg/Abl interacting protein; Arg/Abl-interacting protein 2; OTTHUMP00000211726
Accession O35413

Image & description

Fig.1. Rat Sorbin and SH3 domain-containing protein 2 (SORBS2) Standard Curve.

Fig.1. Rat Sorbin and SH3 domain-containing protein 2 (SORBS2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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