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Rat Solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3) ELISA Kit

Rat Solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3) ELISA Kit

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Specification

Product name Rat Solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC2A3 in samples. An antibody specific for SLC2A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC2A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC2A3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC2A3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC2A3; FLJ90380; GLUT3; glucose transporter type 3; brain; solute carrier family 2; member 3

Product Properties

Kit components • Rat Solute carrier family 2, facilitated glucose transporter member 3 microplate
• Rat Solute carrier family 2, facilitated glucose transporter member 3 standard
• Rat Solute carrier family 2, facilitated glucose transporter member 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLC2A3. No significant cross-reactivity or interference between Rat SLC2A3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background GLUT3 is a high-affinity isoform of Type I glucose transporter expressed mostly in neurons where it is believed to be the main glucose transporter isoform, and in the placenta. GLUTs are integral membrane proteins which contain 12 membrane spanning helices with both the amino and carboxyl termini exposed on the cytoplasmic side of the plasma membrane. GLUT proteins transport glucose and related hexoses according to a model of alternate conformation, which predicts that the transporter exposes a single substrate binding site toward either the outside or the inside of the cell. Binding of glucose to one site provokes a conformational change associated with transport, and releases glucose to the other side of the membrane.
Alternative SLC2A3; FLJ90380; GLUT3; glucose transporter type 3; brain; solute carrier family 2; member 3
Accession Q9XSC2

Image & description

Fig.1. Rat Solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3) Standard Curve.

Fig.1. Rat Solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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