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Rat Solute carrier family 12 member 4 (SLC12A4) ELISA Kit

Rat Solute carrier family 12 member 4 (SLC12A4) ELISA Kit

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Specification

Product name Rat Solute carrier family 12 member 4 (SLC12A4) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Solute carrier family 12 member 4 (SLC12A4) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC12A4 in samples. An antibody specific for SLC12A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC12A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC12A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC12A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC12A4; FLJ17069; FLJ40489; KCC1; K-Cl cotransporter; erythroid K:Cl cotransporter; potassium/chloride cotransporter 1; solute carrier family 12; member 4

Product Properties

Kit components • Rat Solute carrier family 12 member 4 microplate
• Rat Solute carrier family 12 member 4 standard
• Rat Solute carrier family 12 member 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Solute carrier family 12 member 4 (SLC12A4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLC12A4. No significant cross-reactivity or interference between Rat SLC12A4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The full-length sequence was obtained from EST clones and by RT-PCR of HEK293 cell RNA. The 1,085-amino acid KCC1 protein is 24 to 25% identical to NKCC1 and SLC12A3 and shares 97% identity with rabbit KCC1. The overall structure of KCC1 is similar to that of other cation-chloride cotransporters, with 12 predicted transmembrane regions, a large extracellular loop with potential N-linked glycosylation sites, and cytoplasmic N- and C-terminal domains. Northern blot analysis revealed that KCC1 is expressed ubiquitously. KCC1 exhibits the functional properties of the red cell K-Cl cotransporter, including stimulation by swelling and N-ethylmaleimide, and low affinities for rubidium, chloride, and bumetanide.
Gene ID 100009441
Alternative SLC12A4; FLJ17069; FLJ40489; KCC1; K-Cl cotransporter; erythroid K:Cl cotransporter; potassium/chloride cotransporter 1; solute carrier family 12; member 4
Accession Q28677

Image & description

Fig.1. Rat Solute carrier family 12 member 4 (SLC12A4) Standard Curve.

Fig.1. Rat Solute carrier family 12 member 4 (SLC12A4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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