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Rat Soluble endothelial protein C receptor (sEPCR) ELISA Kit

Rat Soluble endothelial protein C receptor (sEPCR) ELISA Kit

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Specification

Product name Rat Soluble endothelial protein C receptor (sEPCR) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Soluble endothelial protein C receptor (sEPCR) ELISA Kit employs a two-site sandwich ELISA to quantitate PROCR in samples. An antibody specific for PROCR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROCR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROCR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROCR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PROCR; CCCA; CCD41; CD201; EPCR; MGC23024; bA42O4.2; APC receptor; CD201 antigen; OTTHUMP00000030729; activated protein C receptor; cell cycle; centrosome-associated protein; centrocyclin; endothelial protein C

Product Properties

Kit components • Rat Soluble endothelial protein C receptor microplate
• Rat Soluble endothelial protein C receptor standard
• Rat Soluble endothelial protein C receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Soluble endothelial protein C receptor (sEPCR) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PROCR. No significant cross-reactivity or interference between Rat PROCR and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Endothelial protein C receptor (EPCR) also known as activated protein C receptor (APC receptor) is a protein that in humans is encoded by the PROCR gene.PROCR has also recently been designated CD201 (cluster of differentiation 201). The protein encoded by this gene is a receptor for protein C that enhances its activation. Protein C is an anti-coagulant serine protease activated by the blood coagulation pathway.
Gene ID 362248
Alternative PROCR; CCCA; CCD41; CD201; EPCR; MGC23024; bA42O4.2; APC receptor; CD201 antigen; OTTHUMP00000030729; activated protein C receptor; cell cycle; centrosome-associated protein; centrocyclin; endothelial protein C
Accession Q4V8I1

Image & description

Fig.1. Rat Soluble endothelial protein C receptor (sEPCR) Standard Curve.

Fig.1. Rat Soluble endothelial protein C receptor (sEPCR) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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