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Rat Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit

Rat Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit

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Specification

Product name Rat Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC5A2 in samples. An antibody specific for SLC5A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC5A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC5A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC5A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC5A2; SGLT2; Na(+)/glucose cotransporter 2; low affinity sodium-glucose cotransporter; solute carrier family 5 (sodium/glucose transporter); member 2

Product Properties

Kit components • Rat Sodium/glucose cotransporter 2 microplate
• Rat Sodium/glucose cotransporter 2 standard
• Rat Sodium/glucose cotransporter 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLC5A2. No significant cross-reactivity or interference between Rat SLC5A2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The major reabsorptive mechanism for D-glucose in the kidney is known to involve a lower affinity, high capacity Na(+)/glucose cotransporter, which is located in the early proximal convoluted tubule segment S1 and has a Na(+)-to-glucose coupling ratio of 1:1.Uptake experiments using cRNA-injected Xenopus oocytes showed specific uptake of glucose and alpha-methyl glucopyranoside at up to three times background. This uptake was saturable, suggesting that the cDNA corresponds to a low-affinity kidney sodium-glucose transporter. It was therefore referred to as sodium-glucose cotransporter 2, or SGLT2. SGLT2 mediates saturable Na(+)-dependent and phlorizin-sensitive transport with K(m) values consistent with low affinity Na(+)/glucose cotransport.
Gene ID 100009386
Alternative SLC5A2; SGLT2; Na(+)/glucose cotransporter 2; low affinity sodium-glucose cotransporter; solute carrier family 5 (sodium/glucose transporter); member 2
Accession P26430

Image & description

Fig.1. Rat Sodium/glucose cotransporter 2 (SLC5A2) Standard Curve.

Fig.1. Rat Sodium/glucose cotransporter 2 (SLC5A2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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