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Rat Slit homolog 1 protein (SLIT1) ELISA Kit

Rat Slit homolog 1 protein (SLIT1) ELISA Kit

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Specification

Product name Rat Slit homolog 1 protein (SLIT1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Slit homolog 1 protein (SLIT1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLIT1 in samples. An antibody specific for SLIT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLIT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLIT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLIT1; RP11-175O19.2; MEGF4; MGC164811; SLIL1; SLIT3; Slit-1; slit homolog 1

Product Properties

Kit components • Rat Slit homolog 1 protein microplate
• Rat Slit homolog 1 protein standard
• Rat Slit homolog 1 protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Slit homolog 1 protein (SLIT1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLIT1. No significant cross-reactivity or interference between Rat SLIT1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The SLIT1 cDNA encodes a 1,534-amino acid polypeptide with 43.5% similarity to the Drosophila 'slit' protein. Northern blot analysis revealed that the human SLIT1 gene was expressed as a major 8.4- and a minor 5.9-kb transcript primarily in the brain. SLIT2 and SLIT3 mRNAs were primarily expressed in the spinal cord and thyroid, respectively. In situ hybridization studies indicated that the rat Slit1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. These data suggested that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.
Gene ID 65047
Alternative SLIT1; RP11-175O19.2; MEGF4; MGC164811; SLIL1; SLIT3; Slit-1; slit homolog 1
Accession O88279

Image & description

Fig.1. Rat Slit homolog 1 protein (SLIT1) Standard Curve.

Fig.1. Rat Slit homolog 1 protein (SLIT1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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