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Rat Serine/threonine-protein kinase TAO1 (TAOK1) ELISA Kit

Rat Serine/threonine-protein kinase TAO1 (TAOK1) ELISA Kit

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Specification

Product name Rat Serine/threonine-protein kinase TAO1 (TAOK1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Serine/threonine-protein kinase TAO1 (TAOK1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAOK1 in samples. An antibody specific for TAOK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAOK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAOK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAOK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TAOK1; FLJ14314; KIAA1361; MAP3K16; MARKK; PSK2; TAO1; STE20-like kinase; microtubule affinity regulating kinase kinase; serine/threonine kinase TAO1; serine/threonine protein kinase TAO1 homolog

Product Properties

Kit components • Rat Serine/threonine-protein kinase TAO1 microplate
• Rat Serine/threonine-protein kinase TAO1 standard
• Rat Serine/threonine-protein kinase TAO1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Serine/threonine-protein kinase TAO1 (TAOK1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TAOK1. No significant cross-reactivity or interference between Rat TAOK1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis: regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade.
Gene ID 286993
Alternative TAOK1; FLJ14314; KIAA1361; MAP3K16; MARKK; PSK2; TAO1; STE20-like kinase; microtubule affinity regulating kinase kinase; serine/threonine kinase TAO1; serine/threonine protein kinase TAO1 homolog
Accession O88664

Image & description

Fig.1. Rat Serine/threonine-protein kinase TAO1 (TAOK1) Standard Curve.

Fig.1. Rat Serine/threonine-protein kinase TAO1 (TAOK1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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