Login Register
English
0

Cart

$ 0

Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit

Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit

Views(130) Publications(0) Catalog no(KTE100472)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit employs a two-site sandwich ELISA to quantitate PKN1 in samples. An antibody specific for PKN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPKN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PKN1; DBK; MGC46204; PAK1; PKN; PKN-ALPHA; PRK1; PRKCL1; protein kinase C-like 1; protein kinase C-like PKN; protein kinase C-related kinase 1; serine-threonine kinase N; serine/threonine protein kinase N

Product Properties

Kit components • Rat Serine/threonine-protein kinase N1 microplate
• Rat Serine/threonine-protein kinase N1 standard
• Rat Serine/threonine-protein kinase N1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PKN1. No significant cross-reactivity or interference between Rat PKN1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Serine/threonine-protein kinase N1 encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene ID 29355
Alternative PKN1; DBK; MGC46204; PAK1; PKN; PKN-ALPHA; PRK1; PRKCL1; protein kinase C-like 1; protein kinase C-like PKN; protein kinase C-related kinase 1; serine-threonine kinase N; serine/threonine protein kinase N
Accession Q63433

Image & description

Fig.1. Rat Serine/threonine-protein kinase N1 (PKN1) Standard Curve.

Fig.1. Rat Serine/threonine-protein kinase N1 (PKN1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit”