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Rat Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit

Rat Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit

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Specification

Product name Rat Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit employs a two-site sandwich ELISA to quantitate DCLK1 in samples. An antibody specific for DCLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCLK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DCLK1; CL1; CLICK1; DCAMKL1; DCDC3A; DCLK; KIAA0369; doublecortin and CaM kinase-like 1

Product Properties

Kit components • Rat Serine/threonine-protein kinase DCLK1 microplate
• Rat Serine/threonine-protein kinase DCLK1 standard
• Rat Serine/threonine-protein kinase DCLK1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DCLK1. No significant cross-reactivity or interference between Rat DCLK1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Dclk1 encodes a member of the protein kinase superfamily and the doublecortin family. The typical protein encoded by Dclk1 contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the protein is independent of its protein kinase activity. Dclk1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the full-length nature of the variant produced from the 5' promoter has not been determined. Current reference sequence data represents two alternatively spliced transcript variants produced from the 3' promoter and their protein products lack the doublecortin domain.
Gene ID 83825
Alternative DCLK1; CL1; CLICK1; DCAMKL1; DCDC3A; DCLK; KIAA0369; doublecortin and CaM kinase-like 1
Accession O08875

Image & description

Fig.1. Rat Serine/threonine-protein kinase DCLK1 (DCLK1) Standard Curve.

Fig.1. Rat Serine/threonine-protein kinase DCLK1 (DCLK1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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