Login Register
English
0

Cart

$ 0

Rat Sepiapterin reductase (SPR) ELISA Kit

Rat Sepiapterin reductase (SPR) ELISA Kit

Views(48) Publications(0) Catalog no(KTE100126)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Rat Sepiapterin reductase (SPR) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Sepiapterin reductase (SPR) ELISA Kit employs a two-site sandwich ELISA to quantitate SPR in samples. An antibody specific for SPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPR; SDR38C1; sepiapterin reductase; short chain dehydrogenase/reductase family 38C; member 1

Product Properties

Kit components • Rat Sepiapterin reductase microplate
• Rat Sepiapterin reductase standard
• Rat Sepiapterin reductase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Sepiapterin reductase (SPR) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SPR. No significant cross-reactivity or interference between Rat SPR and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase; EC 1.1.1.153) catalyzes the NADPH-dependent reduction of various carbonyl substances, including derivatives of pteridines, and belongs to a group of enzymes called aldo-keto reductases. SPR plays an important role in the biosynthesis of tetrahydrobiopterin (BH4). The clone encoded a protein of 261 amino acids with a calculated molecular mass of 28,047 Da. The predicted amino acid sequence of human sepiapterin reductase shows 74% identity with the rat enzyme and a striking homology with human carbonyl reductase, estradiol 17-beta-dehydrogenase, and 3-beta-hydroxy-5-ene steroid dehydrogenase, especially in the N-terminal region.
Gene ID 29270
Alternative SPR; SDR38C1; sepiapterin reductase; short chain dehydrogenase/reductase family 38C; member 1
Accession P18297

Image & description

Fig.1. Rat Sepiapterin reductase (SPR) Standard Curve.

Fig.1. Rat Sepiapterin reductase (SPR) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Rat Sepiapterin reductase (SPR) ELISA Kit”