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Rat Rhodopsin (RHO) ELISA Kit

Rat Rhodopsin (RHO) ELISA Kit

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Specification

Product name Rat Rhodopsin (RHO) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Rhodopsin (RHO) ELISA Kit employs a two-site sandwich ELISA to quantitate RHO in samples. An antibody specific for RHO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRHO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RHO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RHO bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RHO; CSNBAD1; MGC138309; MGC138311; OPN2; RP4; opsin 2; rod pigment; retinitis pigmentosa 4; autosomal dominant; rhodopsin (opsin 2; rod pigment) (retinitis pigmentosa 4; autosomal dominant)

Product Properties

Kit components • Rat Rhodopsin microplate
• Rat Rhodopsin standard
• Rat Rhodopsin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Rhodopsin (RHO) ELISA Kit has high sensitivity and excellent specificity for detection of Rat RHO. No significant cross-reactivity or interference between Rat RHO and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Rhodopsin is a pigment of the retina that is responsible for both the formation of the photoreceptor cells and the first events in the perception of light. Rhodopsins belong to the G-protein coupled receptor family and are extremely sensitive to light, enabling vision in low-light conditions. Exposed to light, the pigment immediately photobleaches, and it takes about 30 minutes to regenerate fully in humans.Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven transmembrane helices, binds retinal, a photoreactive chromophore, in a central pocket. Retinal is produced in the retina from Vitamin A. Isomerization of 11-cis-retinal into all-trans-retinal by light induces a conformational change in opsin that activates the associated G protein and triggers a second messenger cascade.
Gene ID 100009200
Alternative RHO; CSNBAD1; MGC138309; MGC138311; OPN2; RP4; opsin 2; rod pigment; retinitis pigmentosa 4; autosomal dominant; rhodopsin (opsin 2; rod pigment) (retinitis pigmentosa 4; autosomal dominant)
Accession P49912

Image & description

Fig.1. Rat Rhodopsin (RHO) Standard Curve.

Fig.1. Rat Rhodopsin (RHO) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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