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Rat Replication protein A 70 kDa DNA-binding subunit (RPA1) ELISA Kit

Rat Replication protein A 70 kDa DNA-binding subunit (RPA1) ELISA Kit

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Specification

Product name Rat Replication protein A 70 kDa DNA-binding subunit (RPA1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Replication protein A 70 kDa DNA-binding subunit (RPA1) ELISA Kit employs a two-site sandwich ELISA to quantitate RPA1 in samples. An antibody specific for RPA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRPA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RPA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RPA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RPA1; HSSB; MST075; REPA1; RF-A; RP-A; RPA70; MSTP075; replication protein A1

Product Properties

Kit components • Rat Replication protein A 70 kDa DNA-binding subunit microplate
• Rat Replication protein A 70 kDa DNA-binding subunit standard
• Rat Replication protein A 70 kDa DNA-binding subunit detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Replication protein A 70 kDa DNA-binding subunit (RPA1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat RPA1. No significant cross-reactivity or interference between Rat RPA1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Replication protein A (RPA) is a 3-subunit single-stranded DNA (ssDNA)-binding protein that has been isolated from human cells and found to be essential for in vitro replication of the papovavirus SV40. The human cDNA directed production in E. coli of a 70-kD protein that reacted with a monoclonal antibody directed against the 70-kD subunit of the human protein. The recombinant subunit, purified from bacteria, exhibited single-stranded DNA-binding activity comparable to that of the complete RPA complex. It could substitute for the complete complex in stimulating the activity of DNA polymerase alpha-primase, but could not substitute for the complete complex in SV40 DNA replication in vitro, suggesting an important functional role for the other subunits.
Alternative RPA1; HSSB; MST075; REPA1; RF-A; RP-A; RPA70; MSTP075; replication protein A1

Image & description

Fig.1. Rat Replication protein A 70 kDa DNA-binding subunit (RPA1) Standard Curve.

Fig.1. Rat Replication protein A 70 kDa DNA-binding subunit (RPA1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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