|Product name||Rat Regenerating gene 1a (REG-1a) ELISA Kit|
|Applications notes||This Rat Regenerating gene 1a (REG-1a) ELISA Kit employs a two-site sandwich ELISA to quantitate REG-1a in samples. An antibody specific for REG-1a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREG-1a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG-1a is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG-1a bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Regenerating gene 1a microplate
• Rat Regenerating gene 1a standard
• Rat Regenerating gene 1a detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Regenerating gene 1a (REG-1a) ELISA Kit has high sensitivity and excellent specificity for detection of Rat REG-1a. No significant cross-reactivity or interference between Rat REG-1a and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Pancreatic stone protein is the major component of the protein matrix of calculi in patients suffering from chronic calcifying pancreatitis. Secretory pancreatic stone protein is a glycoprotein in the pancreatic secretion. This protein, called PSPS, occurs in multiple molecular forms due to posttranslational processing. The abundance of PSPS in pancreatic juice (10 to 14% of total protein) suggests that it plays an important role in exocrine pancreatic function. In vitro experiments show that PSPS inhibits CaCO(3) crystal growth. Since in all the pancreatic secretions are supersaturated in calcium carbonate, the physiologic role of PSPS may be related to its inhibitory properties. This gene encodes a protein that is secreted by the exocrine pancreas.|
Fig.1. Rat Regenerating gene 1a (REG-1a) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.