Login Register
English
0

Cart

$ 0

Rat Reactive oxygen species modulator 1 (ROMO1) ELISA Kit

Rat Reactive oxygen species modulator 1 (ROMO1) ELISA Kit

Views(141) Publications(0) Catalog no(KTE100977)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Rat Reactive oxygen species modulator 1 (ROMO1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Reactive oxygen species modulator 1 (ROMO1) ELISA Kit employs a two-site sandwich ELISA to quantitate ROMO1 in samples. An antibody specific for ROMO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyROMO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ROMO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ROMO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ROMO1; RP11-353C18.54; C20orf52; MGC111180; MTGMP; bA353C18.2; OTTHUMP00000062052; OTTHUMP00000196684; mitochondrial targeting GXXXG protein

Product Properties

Kit components • Rat Reactive oxygen species modulator 1 microplate
• Rat Reactive oxygen species modulator 1 standard
• Rat Reactive oxygen species modulator 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Reactive oxygen species modulator 1 (ROMO1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ROMO1. No significant cross-reactivity or interference between Rat ROMO1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The main source of ROS is known to be the mitochondria, and increased levels of ROS from the mitochondria have been observed in many cancer cells. Thus far, the mechanism of ROS production in cancer cell proliferation in the mitochondria is not well-understood. Romo1 increased expression of Romo1-triggered ROS production in the mitochondria. The experiments conducted in the present study showed that Romo1-derived ROS were indispensable for the proliferation of both normal and cancer cells. Furthermore, whilst cell growth was inhibited by blocking the ERK pathway in cells transfected with siRNA directed against Romo1, the cell growth was recovered by addition of exogenous hydrogen peroxide. The results of this study suggest that Romo1-induced ROS may play an important role in redox signaling in cancer cells.
Alternative ROMO1; RP11-353C18.54; C20orf52; MGC111180; MTGMP; bA353C18.2; OTTHUMP00000062052; OTTHUMP00000196684; mitochondrial targeting GXXXG protein

Image & description

Fig.1. Rat Reactive oxygen species modulator 1 (ROMO1) Standard Curve.

Fig.1. Rat Reactive oxygen species modulator 1 (ROMO1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Rat Reactive oxygen species modulator 1 (ROMO1) ELISA Kit”