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Rat Ras-related C3 Botulinum Toxin Substrate 1 (RAC1) ELISA Kit

Rat Ras-related C3 Botulinum Toxin Substrate 1 (RAC1) ELISA Kit

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Specification

Product name Rat Ras-related C3 Botulinum Toxin Substrate 1 (RAC1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Ras-related C3 Botulinum Toxin Substrate 1 (RAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate RAC1 in samples. An antibody specific for RAC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RAC1; MGC111543; MIG5; TC-25; p21-Rac1; migration-inducing gene 5; ras-related C3 botulinum toxin substrate 1; rho family; small GTP binding protein Rac1

Product Properties

Kit components • Rat Ras-related C3 Botulinum Toxin Substrate 1 microplate
• Rat Ras-related C3 Botulinum Toxin Substrate 1 standard
• Rat Ras-related C3 Botulinum Toxin Substrate 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Ras-related C3 Botulinum Toxin Substrate 1 (RAC1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat RAC1. No significant cross-reactivity or interference between Rat RAC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. This mutant did bind to POR1 and it induced membrane ruffling and transformation. A second RAC mutant, which bound PAK but not POR1, induced JNK activation but was defective in inducing membrane ruffling and transformation. The authors concluded that the effects of RAC on the JNK cascade and on actin polymerization and cell proliferation are mediated by distinct effector functions that diverge at the level of RAC itself.
Gene ID 363875
Alternative RAC1; MGC111543; MIG5; TC-25; p21-Rac1; migration-inducing gene 5; ras-related C3 botulinum toxin substrate 1; rho family; small GTP binding protein Rac1
Accession Q6RUV5

Image & description

Fig.1. Rat Ras-related C3 Botulinum Toxin Substrate 1 (RAC1) Standard Curve.

Fig.1. Rat Ras-related C3 Botulinum Toxin Substrate 1 (RAC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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