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Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (PDK1) ELISA Kit

Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (PDK1) ELISA Kit

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Specification

Product name Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (PDK1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (PDK1) ELISA Kit employs a two-site sandwich ELISA to quantitate PDK1 in samples. An antibody specific for PDK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PDK1; OTTHUMP00000205076; mitochondrial pyruvate dehydrogenase; lipoamide; kinase isoenzyme 1; pyruvate dehydrogenase kinase 1; pyruvate dehydrogenase kinase; isoenzyme 1

Product Properties

Kit components • Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial microplate
• Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial standard
• Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (PDK1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PDK1. No significant cross-reactivity or interference between Rat PDK1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PDK1 codes for an isozyme of pyruvate dehydrogenase kinase (PDK).The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart. Pyruvate dehydrogenase (PDH) is a part of a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.
Alternative PDK1; OTTHUMP00000205076; mitochondrial pyruvate dehydrogenase; lipoamide; kinase isoenzyme 1; pyruvate dehydrogenase kinase 1; pyruvate dehydrogenase kinase; isoenzyme 1
Accession Q63065

Image & description

Fig.1. Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (PDK1) Standard Curve.

Fig.1. Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (PDK1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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