|Product name||Rat Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1 (PYROXD1) ELISA Kit|
|Applications notes||This Rat Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1 (PYROXD1) ELISA Kit employs a two-site sandwich ELISA to quantitate PYROXD1 in samples. An antibody specific for PYROXD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYROXD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYROXD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYROXD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1 microplate
• Rat Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1 standard
• Rat Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1 (PYROXD1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PYROXD1. No significant cross-reactivity or interference between Rat PYROXD1 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||PYROXD1 Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. In biochemistry, an oxidoreductase is an enzyme that catalyzes the transfer of electrons from one molecule (the reductant, also called the hydrogen or electron donor) to another (the oxidant, also called the hydrogen or electron acceptor). This group of enzymes usually utilizes NADP or NAD as cofactors. Proper names of oxidoreductases are formed as "donor:acceptor oxidoreductase"; however, other names are much more common. The common name is "donor dehydrogenase" when possible, such as glyceraldehyde-3-phosphate dehydrogenase for the second reaction above. Common names are also sometimes formed as "acceptor reductase", such as NAD+ reductase. "Donor oxidase" is a special case where O2 is the acceptor.|
Fig.1. Rat Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1 (PYROXD1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.