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Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

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Specification

Product name Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTPB in samples. An antibody specific for SFTPB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTPB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SFTPB; PSP-B; SFTB3; SFTP3; SMDP1; SP-B; OTTHUMP00000203253; Pulmonary surfactant-associated protein B; 18kD; surfactant; pulmonary-associated protein B

Product Properties

Kit components • Rat Pulmonary Surfactant-associated protein B microplate
• Rat Pulmonary Surfactant-associated protein B standard
• Rat Pulmonary Surfactant-associated protein B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SFTPB. No significant cross-reactivity or interference between Rat SFTPB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SP-B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP-B. Pulmonary surfactant-associated protein B' is a membrane protein which manufactures surfactant.Humans and animals born with a congenital absence of SP-B suffer from intractable respiratory failure. The SFTPB is an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro.
Gene ID 100009194
Alternative SFTPB; PSP-B; SFTB3; SFTP3; SMDP1; SP-B; OTTHUMP00000203253; Pulmonary surfactant-associated protein B; 18kD; surfactant; pulmonary-associated protein B
Accession P15285

Image & description

Fig.1. Rat Pulmonary Surfactant-associated protein B (SP-B) Standard Curve.

Fig.1. Rat Pulmonary Surfactant-associated protein B (SP-B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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