|Product name||Rat Protein kinase C zeta type (PRKCZ) ELISA Kit|
|Applications notes||This Rat Protein kinase C zeta type (PRKCZ) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKCZ in samples. An antibody specific for PRKCZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKCZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKCZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKCZ bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Alternative||PRKCZ; PKC-ZETA; PKC2;|
|Kit components||• Rat Protein kinase C zeta type microplate
• Rat Protein kinase C zeta type standard
• Rat Protein kinase C zeta type detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Protein kinase C zeta type (PRKCZ) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRKCZ. No significant cross-reactivity or interference between Rat PRKCZ and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Protein kinase C, zeta is an enzyme which in humans is encoded by the PRKCZ gene. The PRKCZ gene encodes at least two alternative transcripts, the full-length PKC zeta and an N-terminal truncated form PKM zeta. The truncated PKMζ is thought to be responsible for maintaining long-term memories in the brain.PKC-zeta has an N-terminal regulatory domain, followed by a hinge region and a C-terminal catalytic domain. Second messengers stimulate PKCs by binding to the regulatory domain, translocating the enzyme from cytosol to membrane, and producing a conformational change that removes autoinhibition of the PKC catalytic activity. PKM-zeta, a brain-specific isoform of PKC-zeta generated from an alternative transcript, lacks the regulatory region of full-length PKC-zeta and is therefore constitutively active.|
|Alternative||PRKCZ; PKC-ZETA; PKC2;|
Fig.1. Rat Protein kinase C zeta type (PRKCZ) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.