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Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) ELISA Kit

Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) ELISA Kit

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Specification

Product name Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) ELISA Kit employs a two-site sandwich ELISA to quantitate PCSK6 in samples. An antibody specific for PCSK6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCSK6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PCSK6; PACE4; SPC4; paired basic amino acid cleaving system 4; subtilisin-like proprotein convertase 4; subtilisin-like protease; subtilisin/kexin-like protease PACE4

Product Properties

Kit components • Rat Proprotein convertase subtilisin/kexin type 6 microplate
• Rat Proprotein convertase subtilisin/kexin type 6 standard
• Rat Proprotein convertase subtilisin/kexin type 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PCSK6. No significant cross-reactivity or interference between Rat PCSK6 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Proprotein convertase subtilisin/kexin type 6 is an enzyme that belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease that can cleave precursor protein at their paired basic amino acid processing sites. Each PACE4 isoform exhibits a unique restricted distribution. PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. PACE4A-II is at least expressed in placenta. PACE4B was only found in the embryonic kidney cell line from which it was isolated. PACE4C and PACE4D are expressed in placenta.
Gene ID 25507
Alternative PCSK6; PACE4; SPC4; paired basic amino acid cleaving system 4; subtilisin-like proprotein convertase 4; subtilisin-like protease; subtilisin/kexin-like protease PACE4
Accession Q63415

Image & description

Fig.1. Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) Standard Curve.

Fig.1. Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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