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Rat Prolyl hydroxylases (PHD) ELISA Kit

Rat Prolyl hydroxylases (PHD) ELISA Kit

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Specification

Product name Rat Prolyl hydroxylases (PHD) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Prolyl hydroxylases (PHD) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA1 in samples. An antibody specific for P4HA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP4HA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P4HA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P4HA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative P4HA1; P4HA; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase); alpha polypeptide I; prolyl 4-hydroxylase; alpha I subunit

Product Properties

Kit components • Rat Prolyl hydroxylases microplate
• Rat Prolyl hydroxylases standard
• Rat Prolyl hydroxylases detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Prolyl hydroxylases (PHD) ELISA Kit has high sensitivity and excellent specificity for detection of Rat P4HA1. No significant cross-reactivity or interference between Rat P4HA1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PHF8 belongs to the family of ferrous iron and 2-oxoglutarate dependent oxygenases, and is active as a histone lysine demethylase with selectivity for the di-and monomethyl states. Mutations in PHF8 cause Siderius type X-linked mental retardation (XLMR). In addition to moderate mental retardation, features of the Siderius-Hamel syndrome include facial dysmorphism, cleft lip and/or cleft palate, and in some cases microcephaly. A chromosomal microdeletion on Xp11.22 encompassing all of the PHF8 and FAM120C genes and a part of the WNK3 gene was reported in two brothers with autism spectrum disorder in addition to Siderius-type XLMR and cleft lip and palate.
Gene ID 64475
Alternative P4HA1; P4HA; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase); alpha polypeptide I; prolyl 4-hydroxylase; alpha I subunit
Accession P54001

Image & description

Fig.1. Rat Prolyl hydroxylases (PHD) Standard Curve.

Fig.1. Rat Prolyl hydroxylases (PHD) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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