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Rat Proline-rich nuclear receptor coactivator 1 (PNRC1) ELISA Kit

Rat Proline-rich nuclear receptor coactivator 1 (PNRC1) ELISA Kit

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Specification

Product name Rat Proline-rich nuclear receptor coactivator 1 (PNRC1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Proline-rich nuclear receptor coactivator 1 (PNRC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PNRC1 in samples. An antibody specific for PNRC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNRC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNRC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNRC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PNRC1; B4-2; PNAS-145; PROL2; PRR2; RP11-63L7.5; proline rich 2 protein; proline-rich polypeptide 2; proline-rich protein with nuclear targeting signal

Product Properties

Kit components • Rat Proline-rich nuclear receptor coactivator 1 microplate
• Rat Proline-rich nuclear receptor coactivator 1 standard
• Rat Proline-rich nuclear receptor coactivator 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Proline-rich nuclear receptor coactivator 1 (PNRC1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PNRC1. No significant cross-reactivity or interference between Rat PNRC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Proline-rich nuclear receptor coactivator 1 is a protein encoded by the PNRC1 gene.By screening a natural killer (NK) cell cDNA library subtracted with a Jurkat T-cell line, Chen et al. (1995) obtained a cDNA encoding PROL2, which they termed B4-2. The deduced 327-amino acid proline-rich protein has a potential N-terminal SH3-binding domain, a nuclear targeting sequence, and 7 SPxx or TPxx motifs. Northern blot analysis revealed expression of a 1.9-kb transcript in NK, T, and monocyte cell lines, as well as in a variety of other cell lines. Western blot analysis showed expression of a 35-kD protein. The International Radiation Hybrid Mapping Consortium mapped the PROL2 gene to chromosome 6.
Gene ID 286988
Alternative PNRC1; B4-2; PNAS-145; PROL2; PRR2; RP11-63L7.5; proline rich 2 protein; proline-rich polypeptide 2; proline-rich protein with nuclear targeting signal
Accession Q63647

Image & description

Fig.1. Rat Proline-rich nuclear receptor coactivator 1 (PNRC1) Standard Curve.

Fig.1. Rat Proline-rich nuclear receptor coactivator 1 (PNRC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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