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Rat Proliferating cell nuclear antigen antibody (PCNA-Ab) ELISA Kit

Rat Proliferating cell nuclear antigen antibody (PCNA-Ab) ELISA Kit

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Specification

Product name Rat Proliferating cell nuclear antigen antibody (PCNA-Ab) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Proliferating cell nuclear antigen antibody (PCNA-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCNA-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated PCNA-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled PCNA-Ab and unlabeled PCNA-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PCNA-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PCNA-Ab

Product Properties

Kit components • Rat Proliferating cell nuclear antigen antibody microplate
• Rat Proliferating cell nuclear antigen antibody standard
• Rat Proliferating cell nuclear antigen antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Proliferating cell nuclear antigen antibody (PCNA-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PCNA-Ab. No significant cross-reactivity or interference between Rat PCNA-Ab and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Proliferating Cell Nuclear Antigen a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. It achieves this processivity by encircling the DNA, thus creating a topological link to the genome. It is an example of a DNA clamp. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Alternative PCNA-Ab

Image & description

Fig.1. Rat Proliferating cell nuclear antigen antibody (PCNA-Ab) Standard Curve.

Fig.1. Rat Proliferating cell nuclear antigen antibody (PCNA-Ab) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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