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Rat Prolactin regulatory element-binding protein (PREB) ELISA Kit

Rat Prolactin regulatory element-binding protein (PREB) ELISA Kit

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Specification

Product name Rat Prolactin regulatory element-binding protein (PREB) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Prolactin regulatory element-binding protein (PREB) ELISA Kit employs a two-site sandwich ELISA to quantitate PREB in samples. An antibody specific for PREB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPREB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PREB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PREB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PREB; MGC3467; SEC12; prolactin regulatory binding-element protein; prolactin regulatory element binding protein

Product Properties

Kit components • Rat Prolactin regulatory element-binding protein microplate
• Rat Prolactin regulatory element-binding protein standard
• Rat Prolactin regulatory element-binding protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Prolactin regulatory element-binding protein (PREB) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PREB. No significant cross-reactivity or interference between Rat PREB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Prolactin regulatory element-binding protein is a protein that in humans is encoded by the PREB gene.This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand.
Gene ID 58842
Alternative PREB; MGC3467; SEC12; prolactin regulatory binding-element protein; prolactin regulatory element binding protein
Accession Q9WTV0

Image & description

Fig.1. Rat Prolactin regulatory element-binding protein (PREB) Standard Curve.

Fig.1. Rat Prolactin regulatory element-binding protein (PREB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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