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Rat Prolactin receptor (PRLR) ELISA Kit

Rat Prolactin receptor (PRLR) ELISA Kit

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Specification

Product name Rat Prolactin receptor (PRLR) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Prolactin receptor (PRLR) ELISA Kit employs a two-site sandwich ELISA to quantitate PRLR in samples. An antibody specific for PRLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRLR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRLR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRLR; hPRLrI; delta 4-SF1b truncated prolactin receptor; delta 4-delta 7/11 truncated prolactin receptor; hPRL receptor; prolactin receptor delta 7/11; secreted prolactin binding protein

Product Properties

Kit components • Rat Prolactin receptor microplate
• Rat Prolactin receptor standard
• Rat Prolactin receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Prolactin receptor (PRLR) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRLR. No significant cross-reactivity or interference between Rat PRLR and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The prolactin receptor—encoded by a gene on Chromosome 5p13-14—interacts with the prolactin molecule as a transmembrane receptor.The 5-prime-untranslated region of the PRLR gene contains 2 alternative first exons: E13, the human counterpart of the rat and mouse E13, and a novel human type of alternative first exon termed E1N. The 5-prime-untranslated region also contains a common noncoding exon 2 and part of exon 3, which contains the translation initiation codon. The E13 and E1N exons are within 800 basepairs of each other. These 2 exons are expressed in human breast tissue, breast cancer cells, gonads, and liver. Overall, the transcript containing E13 is prevalent in most tissues. The PRLR gene product is encoded by exons 3-10, of which exon 10 encodes most of the intracellular domain.
Gene ID 100009046
Alternative PRLR; hPRLrI; delta 4-SF1b truncated prolactin receptor; delta 4-delta 7/11 truncated prolactin receptor; hPRL receptor; prolactin receptor delta 7/11; secreted prolactin binding protein
Accession P14787

Image & description

Fig.1. Rat Prolactin receptor (PRLR) Standard Curve.

Fig.1. Rat Prolactin receptor (PRLR) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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