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Rat Proenkephalin-B (PDYN) ELISA Kit

Rat Proenkephalin-B (PDYN) ELISA Kit

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Specification

Product name Rat Proenkephalin-B (PDYN) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Proenkephalin-B (PDYN) ELISA Kit employs a two-site sandwich ELISA to quantitate PDYN in samples. An antibody specific for PDYN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDYN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDYN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDYN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PDYN; MGC26418; PENKB; beta-neoendorphin; beta-neoendorphin-dynorphin; dynorphin; leu-enkephalin; leumorphin; preprodynorphin; preproenkephalin B; rimorphin

Product Properties

Kit components • Rat Proenkephalin-B microplate
• Rat Proenkephalin-B standard
• Rat Proenkephalin-B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Proenkephalin-B (PDYN) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PDYN. No significant cross-reactivity or interference between Rat PDYN and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Prodynorphin is a opioid polypeptide hormone involved with chemical signal transduction and cell communication. The gene for prodynorphin is expressed in the endometrium and the striatum, and its gene map locus is 20pter-p12. Prodynorphin is a basic building block of endorphins, the chemical messengers in the brain that appear most heavily involved in the anticipation and experience of pain and the formation of deep emotional bonds, and which are also critical in learning and memory. The prodynorphin enzyme is identical in humans and chimps, but the regulatory promoter sequences have been shown to exhibit marked differences.The gene is thought to influence perception, as well as susceptibility to drug dependence, and is expressed more readily in human beings than in other primates.
Alternative PDYN; MGC26418; PENKB; beta-neoendorphin; beta-neoendorphin-dynorphin; dynorphin; leu-enkephalin; leumorphin; preprodynorphin; preproenkephalin B; rimorphin
Accession P06300

Image & description

Fig.1. Rat Proenkephalin-B (PDYN) Standard Curve.

Fig.1. Rat Proenkephalin-B (PDYN) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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