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Rat Pro-opiomelanocortin (POMC) ELISA Kit

Rat Pro-opiomelanocortin (POMC) ELISA Kit

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Specification

Product name Rat Pro-opiomelanocortin (POMC) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Pro-opiomelanocortin (POMC) ELISA Kit employs a two-site sandwich ELISA to quantitate POMC in samples. An antibody specific for POMC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOMC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POMC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POMC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative POMC; ACTH; CLIP; LPH; MSH; NPP; POC; OTTHUMP00000119991; OTTHUMP00000200964; adrenocorticotropic hormone; adrenocorticotropin; alpha-MSH; alpha-melanocyte-stimulating hormone; beta-LPH; beta-MSH; beta-endorphin;

Product Properties

Kit components • Rat Pro-opiomelanocortin microplate
• Rat Pro-opiomelanocortin standard
• Rat Pro-opiomelanocortin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Pro-opiomelanocortin (POMC) ELISA Kit has high sensitivity and excellent specificity for detection of Rat POMC. No significant cross-reactivity or interference between Rat POMC and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Pro-opiomelanocortin (POMC) is a precursor polypeptide with 241 amino acid residues. This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products.
Alternative POMC; ACTH; CLIP; LPH; MSH; NPP; POC; OTTHUMP00000119991; OTTHUMP00000200964; adrenocorticotropic hormone; adrenocorticotropin; alpha-MSH; alpha-melanocyte-stimulating hormone; beta-LPH; beta-MSH; beta-endorphin;
Accession P06297

Image & description

Fig.1. Rat Pro-opiomelanocortin (POMC) Standard Curve.

Fig.1. Rat Pro-opiomelanocortin (POMC) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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