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Rat Presenilin 2 (PSEN2) ELISA Kit

Rat Presenilin 2 (PSEN2) ELISA Kit

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Product name Rat Presenilin 2 (PSEN2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Presenilin 2 (PSEN2) ELISA Kit employs a two-site sandwich ELISA to quantitate PSEN2 in samples. An antibody specific for PSEN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSEN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSEN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSEN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PSEN2; AD3L; AD4; PSEN2; STM2; Alzheimer s disease 3-like; presenilin 2

Product Properties

Kit components • Rat Presenilin 2 microplate
• Rat Presenilin 2 standard
• Rat Presenilin 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Presenilin 2 (PSEN2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PSEN2. No significant cross-reactivity or interference between Rat PSEN2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Presenilin-2 is a protein that is encoded by the PSEN2 gene. Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Two alternative transcripts of PSEN2 have been identified. In melanocytic cells PSEN2 gene expression may be regulated by MITF.
Gene ID 81751
Alternative PSEN2; AD3L; AD4; PSEN2; STM2; Alzheimer s disease 3-like; presenilin 2
Accession O88777

Image & description

Fig.1. Rat Presenilin 2 (PSEN2) Standard Curve.

Fig.1. Rat Presenilin 2 (PSEN2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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